A conserved threonine spring-loads precursor for intein splicing
نویسندگان
چکیده
منابع مشابه
An Engineered Split Intein for Photoactivated Protein Trans-Splicing
Protein splicing is mediated by inteins that auto-catalytically join two separated protein fragments with a peptide bond. Here we engineered a genetically encoded synthetic photoactivatable intein (named LOVInC), by using the light-sensitive LOV2 domain from Avena sativa as a switch to modulate the splicing activity of the split DnaE intein from Nostoc punctiforme. Periodic blue light illuminat...
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Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology and biotechnology. Herein, we describe the use of a consensus design approach to engineer a split intein with enhanced stability and activity that make it more robust than any known PTS system. Using batch mutagenesis, we first conduct a detailed analysis of the difference in splicing rates between the Np...
متن کاملBranching out of the intein active site in protein splicing.
Inteins perform a macromolecular vanishing act that continues to impress as its secrets are revealed. Dispersed through all domains of life, these autocatalytic domains exit the folds of ostensibly unrelated proteins soon after translation. Inteins bust at the seams, severing peptide bonds that immediately precede and follow their sequence. Critically, they also sew up the damage; the two polyp...
متن کاملIntramolecular disulfide bond between catalytic cysteines in an intein precursor.
Protein splicing is a self-catalyzed and spontaneous post-translational process in which inteins excise themselves out of precursor proteins while the exteins are ligated together. We report the first discovery of an intramolecular disulfide bond between the two active-site cysteines, Cys1 and Cys+1, in an intein precursor composed of the hyperthermophilic Pyrococcus abyssi PolII intein and e...
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Protein sequences are diversified on the DNA level by recombination and mutation and can be further increased on the RNA level by alternative RNA splicing, involving introns that have important roles in many biological processes. The protein version of introns (inteins), which catalyze protein splicing, were first reported in the 1990s. The biological roles of protein splicing still remain elus...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2013
ISSN: 0961-8368
DOI: 10.1002/pro.2236